This guidance has been developed to ensure proper disposal of ethidium bromide and other common fluorescent dyes (Gels and Solutions), which are commonly used in electrophoresis for the identification of DNA. Ethidium bromide is the most commonly used dye for this type of DNA work and the most toxic. Other common fluorescent dyes used in electrophoresis are; propidium iodide, acridine orange, SYBR Green, etc. When these materials become waste, they are managed by the same guidelines as ethidium bromide wastes.
Note: Electrophoresis wastes containing acrylamide or polyacrylamide must be managed as hazardous waste, as per the Laboratory Hazardous Waste Disposal Policy and Procedures
- Ethidium Bromide Waste Disposal Guidance Document [pdf]
- Ethidium Bromide Waste Disposal Flow Chart [pdf]
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